超净台工作说明书
发布时间:2014/7/21 14:43:00产品说明书
超净工作台的优点是操作方便自如,比较舒适,工作效率高,预备时间短,开机30分钟以上即可操作,基本上可随时使用。在工厂化生产中,接种工作量很大,需要经常长久地工作时,超净台是很理想的设备。超净工作台由三相电机作鼓风动力,功率145~260W左右,将空气通过由特制的微孔泡沫塑料片层叠合组成的“超级滤清器”后吹送出来,形成连续不断的无尘无菌的超净空气层流,即所谓“高效的特殊空气”,它除去了大于0.3μm的尘埃、真菌和细菌孢子等等。超净空气的流速为24~30m/min,这已足够防止附近空气可能袭扰而引起的污染,这样的流速也不会妨碍采用酒精灯或本生灯对器械等的灼烧消毒。工作人员就在这样的无菌条件下操作,保持无菌材料在转移接种过程中不受污染。但是万一操作中途遇到停电,暴露在未过滤空气中的材料便难以幸免污染。这时应迅速结束工作,并在瓶上作出记号,内中的材料如处于增殖阶段,则以后不再用作增殖而转入生根培养。如为一般性生产材料,因极其丰富也可弃去。如处于生根过程,则可留待以后种植用。
超净台电源多采用三相四线制,其中有一零线,连通机器外壳,应接牢在地线上,另外三线都是相线,工作电压是380V。三线接入电路中有一定的顺序,如线头接错了,风机会反转,这时声音正常或稍不正常,超净台正面无风(可用酒精灯火焰观察动静,不宜久试),应及时切断电源,只要将其中任何两相的线头交换一下位置再接上,就可解决。三相线如只接入两相,或三相中有一相接触不良,则机器声音很不正常,应立即切断电源仔细检修,否则会烧毁电机。这些常识应在开始使用超净台时就向工作人员讲解清楚,免除不应造成的事故与损失。
超净工作台进风口在背面或正面的下方,金属网罩内有一普通泡沫塑料片或无纺布,用以阻拦大颗粒尘埃,应常检查、拆洗,如发现泡沫塑料老化,要及时更换。除进风口以外,如有漏气孔隙,应当堵严,如贴胶布,塞棉花,贴胶水纸等。工作台正面的金属网罩内是超级滤清器,超级滤清器也可更换,如因使用年久,尘粒堵塞,风速减小,不能保证无菌操作时,则可换上新的。
超净台使用寿命的长短与空气的洁净程度有关。在温带地区超净台可在一般实验室使用,然而在热带或亚热带地区,由于大气中含有高量的花粉,或多粉尘的地区,超净台则宜放在较好的有双道门的室内使用。任何情况下不应将超净台的进风罩对着开敞的门或窗,以免影响滤清器的使用寿命。
无菌室应定期用70%酒精或0.5%苯酚喷雾降尘和消毒,用2%新洁尔灭抹拭台面和用具(70%酒精也可),用福尔马林(40%甲醛)加少量高锰酸钾定期密闭熏蒸,配合紫外线灭菌灯(每次开启15分钟以上)等等消毒灭菌手段,以使无菌室经常保持高度的无菌状态。接种箱内部也应装有紫外线灯,使用前开灯15分钟以上照射灭菌,但凡是照射不到之处仍是有菌的。在紫外线灯开启时间较长时,可激发空气中的氧分子缔合成臭氧分子,这种气体成分有很强的杀菌作用,可以对紫外线没有直接照到的角落产生灭菌效果。由于臭氧有碍健康,在进入操作之前应先关掉紫外线灯,关后十多分钟即可入内 。
在超净工作台上亦可吊装紫外线灯,但应装在照明灯罩之外,并错开照明灯平行排列,这样在工作时不妨碍照明。若将紫外线灯装入照明灯罩(玻璃板)里面,这是毫无用处的,因为紫外线不能穿透玻璃,它的灯管是石英玻璃,而不是硅酸盐玻璃制成的。
Clean
Bench has the advantage of easy to operate freely, more comfortable, efficient,
preparation time is short, the boot can be operated for more than a very
minute, basically ready to use. In factory production, inoculation heavy
workload, the need for frequent long-term work, the Clean Bench is the ideal
equipment. Clean Bench by the three-phase motor to make blast power, power 145
~ 260W or so, will air through the porous foam by a specially composed piece
cascading together a "super filter" after blowing out the formation
of a continuous dust-free The ultra-clean air laminar flow bacteria, so-called
"high-performance special air", which removed more than 0.3μm dust,
fungi and bacteria spores and so on. Ultra-clean air flow rate of 24 ~30m/min, which are adequate to prevent
possible harassment in the vicinity of air pollution caused by such a flow will
not impede the use of alcohol lamp or Bunsen burner burning disinfection of
equipment and so on. And in this way the staff operated under sterile
conditions to maintain sterile material in the course of the transfer of
vaccination is not contaminated. But what if an electrical outage halfway
operation, exposed to unfiltered air contaminated material will not be spared.
Should provide a speedy end to work and make a mark on the bottle, which is in
the materials, such as the proliferation phase, we will no longer be used as a
proliferation and transferred to rooting culture. As for the general production
of materials, due to the extremely rich can also be disposable. As in the
rooting process, can be planted until later use.
Clean Bench power supply to use more three-phase four-wire
system, of which 10 lines, connecting the machine casing should then firmly on
the ground line, the other three lines are the phase, the working voltage is
380V. Third-line access to the circuit in a certain order, such as pick the
wrong end of a thread, wind the opportunity to reverse, when the sound normal
or slightly abnormal, Clean Bench front without wind (available alcohol lamp
flame observation of static and dynamic, not long-trial), should be promptly
cut off the power, as long as any of them as two-phase end of a thread to
exchange position were followed, the will be over. Three-phase line, such as
access only to two-phase or three-phase there is a similar connection is bad,
then the machine sounds very normal, should be immediately cut off the power
supply careful maintenance, otherwise they will burn motor. These common sense
should be when they start using Clean Bench to explain clearly to staff, should
not result in exemption from the accident and loss.
Clean Bench at the back or front air inlet below the metal
mesh cover within a normal foam piece or non-woven fabrics used to block large
particles of dust, should be a routine check, unpick and wash, such as found in
foam aging, must be promptly replaced. In addition to outside air inlet, where
gas leakage pores, it should be blocked strict, such as patching, plug of
cotton and paste glue paper. Table inside the front cover of the metal mesh
filter is super, super filter may be replaced, as a result of the use of long
years, dust jam, wind speed decreased, can not guarantee aseptic operation, can be replaced with new.
Clean Bench length of life related to the degree of air
cleanliness. Clean Bench in temperate regions may be in the general laboratory
use, but in tropical or subtropical regions, because the atmosphere contains
high levels of pollen, dust or more areas, Clean Bench are advised to put a
better interior double doors. Any case, Taiwan should not be ultra-clean air
inlet hood facing the open door or window, so as not to affect the filter life.
Sterile room should be on a regular basis with 70% alcohol
or 0.5% phenol spray dust and disinfected with 2% wiped countertops and
appliances Bromogeramine (70% alcohol can also be), with formalin (40%
formaldehyde) plus a small amount of high manganese airtight fumigation
potassium on a regular basis to meet the ultraviolet sterilization lamps (each
to open more than 15 minutes) and so on means of disinfection and
sterilization, sterile rooms in order to maintain regular high level of
sterility. Vaccination should also be equipped with a UV box inside lights,
turn on the lights more than 15 minutes prior to use radiation sterilization,
but not of the Department any exposure there is still bacteria. UV light to
open longer, it can stimulate oxygen molecules in the air to enter the ozone
molecule synthesis, this gas composition has a strong bactericidal effect, you
can not directly shine on the corner of ultraviolet sterilizing effect
produced. Because ozone unhealthy, on entering the operation should be preceded
by turn off the UV lamp off after more than 10 minutes you can enter.
In the ultra-clean the table can also be hoisting UV
ultraviolet light, but it should be packed in light shade outside, and stagger
the lights arranged in parallel, so that at work, without prejudice to
lighting. Ruoqiang ultraviolet light into light shade (glass) inside, which is
useless, since ultraviolet light can not penetrate the glass, it is a quartz
glass tube, rather than made of silicate glass.
使用方法
超净工作台内的小环境可被紫外灯消毒,并由高效过滤器鼓风系统使台内形成一个较稳定的无菌环境。工作中人的活动要尽量少改变台内的空气循环系统。
主体内容:
1. 目的 规范净化工作台操作与维护工作,确保仪器正常运作。
2. 适用范围:本实验方法适用于微生物检验中净化工作台操作与维护管理。
3. 检验人员职责:负责此仪器操作和维护管理
4. 操作及维护规程
4.1操作规程
4.1.1使用工作台时,应提前30分钟开机,同时开启紫外杀菌灯,处理操作区内表面积累的微生物,30分钟后关闭杀菌灯(此时日光灯即开启),启动风机。
4.1.2对新安装的或长期未使用的工作台,使用前必需对工作台和周围环镜先用超静真空吸尘器或用不产生纤维的工具进行清洁工作,再采用药物灭菌法或紫外线灭菌法进行灭菌处理。
4.1.3 操作区内不允许存放不必要的物品,保持工作区的洁净气流流型不受干扰。
4.1.4 操作区内尽量避免作用明显扰乱气流流型的动作。
4.1.5 操作区的使用温度不可以超过60℃。
4.2 维护规程及维护方法
4.2.1 根据环境的洁净程度,可定期(一般2~3个月)将粗滤布(涤纶无纺布)拆下清洗或给予更换。
4.2.2 定期(一般为一周)对环境周围进行灭菌工作,同时经常用纱布沾酒精或丙酮等有机溶剂将紫外线杀菌灯表面擦干净,保持表面清洁,否则会影响杀菌效果。
4.2.3 当加大风机电压已不能使风速达到0.32m/s时必须更换高效空气过滤器。
4.2.4 更换过滤器时,可打开顶盖,更换时应注意过滤器上的箭头标志,箭头指向即为层流气流向。
4.2.5 更换高效过滤器后,应用Y09-4型尘埃粒子计数器四周边框密封是否良好,调节风机电压,使操作区平均风速保持在0.32-0.48m/s范围内,再用Y09-4型尘埃粒子计数器检查洁净度
使用管理
1 目的: 维持屏障系统的生物洁净度,保护工作人员的安全,保障机组的正常运转,尽量减少可影响科研成果的各种可变因素。
2 原则: 屏障系统内的超净工作台只能用于建立动物模型的手术、转基因小鼠的日常操作。
3 使用管理:
(1)每次使用超净工作台时,实验人员应先开启超净工作台上的紫外灯,紫外照射30分钟后使用。
(2)开启超净工作台工作电源,关闭紫外灯,并用75%的酒精或0.5%过氧乙酸喷洒擦拭消毒工作台面。
(3)整个实验过程过程中,实验人员应按照无菌操作规程操作。
(4)实验结束后,用消毒液擦拭工作台面,关闭工作电源,重新开启紫外灯照射15分钟。
(5)如遇机组发生故障,应立即通知实验动物室,由人员检修合格后继续使用。
(6)实验人员应注意保持室内整洁。
(7)超净工作台的滤材每2年更换,并作好更换记录。
日常维护
超净工作台是一台较精密的电气设备,对其进行经常性的保养和维护是非常重要的。
首先要保持室内的干燥和清洁,潮湿的空气既会使制造材料锈蚀,还会影响电气电路的正常工作,潮湿空气还利于细菌、霉菌的生长。清洁的环境还可延长滤板的使用寿命。
另外,定期对设备的清洁是正常使用的重要环节。清洁应包括使用前后的例行清洁和定期的熏蒸处理。熏蒸时,应将所有缝隙完全密封。清洁应包括使用前后的例行清洁和定期的熏蒸处理。熏蒸时,应将所有缝隙完全密封,如操作口设有可移动挡板封盖的类型超净工作台,可用塑料薄膜密封。
超净工作台的滤板和紫外杀菌灯都有标定的使用年限,应按期更换。
操作程序
1.超净工作台操作使用前的准备工作
(1)开启超净工作台风机,净化台内环境。打开工作灯达到工作照度。
(2)检查超净工台中准备的各种灭菌饲养和实验物品是否齐全。若需更换笼盒。检查灭菌笼盒准备情况,并用不锈钢推车移至超净台移门边。
(3)检查喷雾器喷洒效果及超净台内外擦抹用的药液是否已配置好。
(4)打开房间内排风系统。排除打开笼盒时逸出的有味气体。
(5)检查IVC机组各种表头指针及目视各笼盒内的动物情况,估测IVC运行情况。
(6)记录影响实验动物繁殖、生长、发育的主要环境参数,如温度、相对湿度、日温差、笼盒内外压差等。
2.超净工作台操作使用程序及规范
(1)人员带好性口罩、帽子及医用乳胶手套。。
(2)用双手轻轻抬起IVC笼盒外端,沿笼架搁挡向外移出笼盒,放在超净工作台旁的不锈钢小推车上。
(3)用药液喷雾器充分喷洒双手手套外表及IVC笼盒的外面,消毒灭菌并沾粘住表面的尘埃,防止进入超净台时被层流气体吹扬。也可用带手套的双手浸入药液容器中,捞起并拧干药液毛巾,擦干手套外液滴,同时擦一下笼盒的外表。
(4)打开超净工作台移门,把笼盒移入超净工作台。
(5)适当拉下超净工作台移门。打开IVC笼盒盖底之间的紧固扣并打开笼盖。侧卧放在一边。
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