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NUCLEODUR C18 Gravity液相色谱柱

价格：电议

地区：北京市

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NUCLEODUR C18 Gravity液相色谱柱

C18Gravity：PH稳定应用范围宽达1.0—11.0

※     满足您对方法开发
※     端PH应用的需求
※     和复杂样品的分离

Gravity是在NUCLEODUR纯硅胶基础上，采用 18%高碳载量和多步交联键合技术。适合强碱性样品的分析，而且能在PH=1.0—11.0范围内稳定使用,合美国药典USP L1，常卖货号760101.46C18 Gravity 4.6X250mm 5um

性能：

主要特点：
适用于液相色谱/质谱和液相色谱法在PH（PH值1 - 11）
的基失活
方法开发的理想
技术特点：
十八烷基改性高密度、多封端
孔径110A；粒径1.8μm，3 m和5 mμ
10，7，12和16μm粒子的制备要
碳含量18%
推荐应用：
整体复杂的分析分离
复合类分离包括，如镇痛药、药、抑郁药；除草剂；植物；免疫抑制剂

*Standard program of columns packed with NUCLEODUR**? C18 Gravity
Column system	粒径:	1.8 μm	3 μm	5 μm	10 μm
分析柱	EC columns (standard system, 2–4.6 mm)		+	+	+	+
制备	VarioPrep columns (VP, ≥ 8 mm)				+	+

液相色谱分析法

订货信息：


Length ->	30 mm	50 mm	75 mm	100 mm	125 mm	150 mm	250 mm
NUCLEODUR? C18 Gravity, 1.8 μm
2 mm ID	760078.20	760079.20	760071.20	760076.20		760075.20
3 mm ID	760078.30	760079.30		760076.30
4 mm ID	760078.40	760079.40		760076.40
4.6 mm ID	760078.46	760079.46		760076.46
EC guard columns*	4 x 2 mm	761901.20		4 x 3 mm	761901.30
NUCLEODUR? C18 Gravity, 3 μm
2 mm ID		760080.20		760084.20	760081.20	760083.20	760082.20
3 mm ID		760080.30		760084.30	760081.30	760083.30	760082.30
4 mm ID		760080.40		760084.40	760081.40	760083.40	760082.40
4.6 mm ID		760080.46	760086.46	760084.46	760081.46	760083.46	760082.46
EC guard columns*	4 x 2 mm	761902.20		4 x 3 mm	761902.30
NUCLEODUR? C18 Gravity, 5 μm
2 mm ID		760102.20		760104.20	760100.20	760103.20	760101.20
3 mm ID		760102.30		760104.30	760100.30	760103.30	760101.30
4 mm ID		760102.40		760104.40	760100.40	760103.40	760101.40
4.6 mm ID		760102.46	760106.46	760104.46	760100.46	760103.46	760101.46
EC guard columns*	4 x 2 mm	761903.20		4 x 3 mm	761903.30

Guard columns for EC columns		EC columns with ID	Use guard column holder
			2 mm	3 mm	4 mm	4.6 mm
*	Column Protection System	EC guard column	4/2	4/3	4/3	4/3	REF 718966

VarioPrep 柱用在液相制备

Length	10 mm ID	21 mm ID	32 mm ID	40 mm ID
NUCLEODUR? C18 Gravity, 5 μm
50 mm	762103.100	762103.210
125 mm	762109.100	762109.210
150 mm				762100.400
250 mm	762113.100	762113.210	762113.320	762113.400
NUCLEODUR? C18 Gravity, 10 μm
250 mm		762250.210		762250.400
VP guard columns with NUCLEODUR? C18 Gravity
	8 mm ID	16 mm ID	32 mm ID
10 mm*	762160.80	762160.160
15 mm*			762163.320

* VP guard column	Size	Pack of	for VP column	required holder	REF
VP 10/8	10 x 8 mm ID	2	10 mm ID	8 mm	718251
VP 10/16	10 x 16 mm ID	2	21 mm ID	16 mm	718256
VP 15/32	15 x 32 mm ID	1	32–40 mm ID	32 mm	718253

对照品舒巴坦钠的标定

使用NUCLEODUR C18 Gravity和对照柱X按照《中国药典》方法检测进行标定

色谱柱：NUCLEODUR C18 Gravity 250×4.6mm 5um 对照柱X 250×4.6mm 5um

流动相：0.005 mol/L 四丁基氢氧化铵（PH=5.0）:乙腈=75:25

温度：35℃

检测器：UV 230nm

结果:

	舒巴坦钠理论塔板数	舒巴坦钠与杂质分离度	重现性
C18 Gravity	7398	4.13	0.71
对照柱X	6317	4.00	0.15

应用：

1、万古霉素

Substances:		vancomycin
Column:		EC 250/4.6 NUCLEODUR^? C18 Gravity, 5 μm
Phase:		NUCLEODUR^? C18 Gravity
REF number:		760101.46

Sample pretreatment:		Sample concentration: 2 mg/mL in eluent A Stock solution: 4 mL triethylamine + 1996 mL water + H₃PO₄ (adjusted to pH 3.2)

Conditions:		Eluent A: 920 mL stock solution + 10 mL THF + 70 mL ACN Eluent B: 700 mL stock solution + 10 mL THF + 290 mL ACN Gradient: 0 % B for 13 min, 0-100 % B in 9.1 min, 100 % B for 4 min Flow rate: 1.0 mL/min Temperature: 25 °C Injection volume: 20 μL

Detection:		UV, 280 nm

Author(-s):		HPLC department
Source:		MACHEREY-NAGEL, Germany 2010

Keywords:		antibiotics; pharmacopeia; Pharm. Eu.; asymmetry factor

Legend:		1 - vancomycin
Note:		Vancomycin is determined with an asymmetry factor of 1.12 (Pharm. Eu< 1.60)

Substances:		acetylsalicylic acid; sulindac; piroxicam; suprofen; tolmetin; naproxen; diflunisal; fenoprofen; flurbiprofen; niflumic acid; indomethacin; ibuprofen
Column:		EC 250/4.6 NUCLEODUR^? C18 Gravity, 5 μm
Phase:		NUCLEODUR^? C18 Gravity
MN catalog number:		760101.46



Conditions:		Mobile phase: acetonitrile / 20 mM KH₂PO₄, pH 2.5, 45:55 (v/v) Flowrate: 1.3 ml/min Temperature: 22 °C Injection volume: 5.0 μl

Detection:		UV, 230 nm

Author(-s):		HPLC department
Source:		MACHEREY-NAGEL, Germany 2008

Keywords:		antiinflamatoray drugs; drugs; NSAID

Legend:

1   - acetylsalicylic acid
2   - sulindac
3   - piroxicam
4   - suprofen
5   - tolmetin
6   - naproxen
7   - diflunisal
8   - fenoprofen
9   - flurbiprofen
10 - niflumic acid
11 - indomethacin
12 - ibuprofen

Substances:		coumafuryl; warfarin; pindone; coumatetralyl; coumachlor; diphacinone; chlorphacinone; bromadiolone i; difenacoum; bromadiolone ii; flocoumafen i; flocumafen ii; brodifacoum; difethialone; pyranocoumarin
Column:		EC 250/4.6 NUCLEODUR^? C18 Gravity, 5 μm CC 8/4 NUCLEODUR^? C18 Gravity, 5 μm (guard column)
Phase:		NUCLEODUR^? C18 Gravity
REF number:		760101.46 761125.40



Conditions:		Buffer: 0.68 g KH₂PO₄ + 0.34 g Na₃PO₄ x 12 H₂0 in 1l dest. water, pH 6.3 Eluents: A: Methanol / Acetonitrile / Buffer (250:100:650) B: Methanol / Acetonitrile / Buffer (100:650:250) Flow rate: 1.2 ml/min Temperature: RT

Detection:		A) DAD UV, 308 nm (285 nm, 260 nm) B) Fluorescence (stimulation 308 nm / emission 390 nm)

Author(-s):		Freidhof, K.
Source:		LandesuntersuchungsamtRheinland-Pfalz, Koblenz; Germany

Keywords:		anti-coagulant drugs; drugs

Legend:

Peaks:

A)
1) Coumafuryl
2) Warfarin &Pindone
3) Coumatetralyl
4) Coumachlor
5) Diphacinone
6) Chlorphacinone
7) Bromadiolone I
8) Difenacoum
9) Bromadiolone II
10) Flocoumafen I
11) Flocumafen II &Brodifacoum
12) Difethialone
13) Pyranocoumarin

B)
1) Coumafuryl
2) Warfarin
3) Coumatetralyl
4) Coumachlor
5) Bromadiolone I
6) Difenacoum
7) Bromadiolone II
8) Flocoumafen&Brodifacoum
9) Pyranocoumarin